crude membrane meaning in English

粗膜

After stimulation of insulin > epinephrine in 10 ~ 6m to the differentiated 3t3 - l1 cells for 48h , or incubated with 16 . 8mm glucose for 48h , the crude membrane proteins were extracted , then investigated the effects of various factors on aqpap protein expression by western blotting . the subjects were divided into three groups according to body mass index : the normal , the overweight , the obese . the expression of adipose aqpap were detected with rt - pcr and western blotting
2 、利用半定量rt - pcr及westernblotting技术，从基因及蛋白质表达水平研究激素(胰岛素、肾上腺素、地塞米松、 igf - 1 ) 、营养物质(葡萄糖、甘油) 、调脂药(非诺贝特、洛伐他汀)对3t3 ? l1脂肪细胞aqpap表达的影响，并观察不同体重研究对象脂肪组织aqpap表达量的变化。